Temporary

Part:BBa_J70664:Design

Designed by: Julie Norville   Group: Knight Lab   (2010-12-29)


Test assembly for Grant's parts


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1933
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4718
    Illegal AgeI site found at 649
    Illegal AgeI site found at 761
    Illegal AgeI site found at 4914
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

First create J70638, then digest M12100 with ES and J70638 with EX. Make sure J70638 and M12100 are on different vectors. (For this method there should be M12100 on all three vectors.) Ligate cut fragments together. Red versions of this on the antibiotic resistance of J70638 part should indicate that the assembly occurred properly. Count ratio and sequence to check.


References